期刊
CHEMICAL COMMUNICATIONS
卷 50, 期 22, 页码 2958-2961出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc48216b
关键词
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资金
- KAKENHI [of the Ministry of Education, Culture, Sports, Science, and Technology of Japan/Japan Society for the Promotion of Science] [21750073, 23658285, 24658049]
- Kurata Memorial Hitachi Science and Technology Foundation
- SECOM Science and Technology Foundation
- Grants-in-Aid for Scientific Research [21750073, 24655056, 23658285, 24658049, 23570088] Funding Source: KAKEN
To date, biochemical approaches to membrane receptors have been limited to the following methods: knockout or overexpression of membrane receptors by gene introduction and genome engineering or extraction of membrane receptor-surfactant complexes from innate cells and their introduction into model biomembranes. Here, we describe the development of a third method involving gene expression using cell-free in situ protein synthesis inside model biomembrane capsules. We verified this method by synthesizing olfactory receptors from the silkmoth Bombyx mori inside giant vesicles and found that they were excited in the presence of their ligand the Bombyx mori sex pheromone.
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