4.7 Article

Cytosine-bulge-dependent fluorescence quenching for the real-time hairpin primer PCR

期刊

CHEMICAL COMMUNICATIONS
卷 50, 期 96, 页码 15195-15198

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/c4cc06780k

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资金

  1. JSPS [23241073, 24310165]
  2. Advanced Research for Medical Products Mining Program of the National Institute of Biomedical Innovation [10-22]
  3. Adaptable and Seamless Technology Transfer Program through target driven R&D (A-STEP) from Japan Science and Technology Agency, JST
  4. Grants-in-Aid for Scientific Research [26000007, 23241073] Funding Source: KAKEN

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The progress of a polymerase chain reaction (PCR) was sensitively monitored based on the increase in fluorescence of N, N'-bis(3-aminopropyl)- 2,7-diamino-1,8-naphthyridine, which was covalently anchored on the cytosine bulge directly neighbouring the 5'-T_G-3'/5'-CCA-3' sequence in the hairpin tag at the 5' end of the PCR primer.

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