期刊
CHEMICAL COMMUNICATIONS
卷 49, 期 65, 页码 7243-7245出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc43224f
关键词
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资金
- National Basic Research Program of China [2010CB732402, 2013CB933703]
- National Science Foundation of China [21205100, 21275122]
- National Instrumentation Program [2011YQ03012412]
- Natural Science Foundation of Fujian Province for Distinguished Young Scholars [2010J06004]
Based on backbone-modified molecular beacons and duplex-specific nuclease, we have developed a target recycling amplification method for highly sensitive and selective miRNA detection. The combination of a low fluorescence background of 2-OMe-RNA modified MB and nuclease-assisted signal amplification leads to ultrahigh assay sensitivity, and the powerful discriminating ability of MB enables the differentiation of highly similar miRNAs with one-base difference, both of which are of great significance to miRNA detection.
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