4.7 Article

A label-free supersandwich electrogenerated chemiluminescence method for the detection of DNA methylation and assay of the methyltransferase activity

期刊

CHEMICAL COMMUNICATIONS
卷 49, 期 37, 页码 3869-3871

出版社

ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc00203a

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资金

  1. National Natural Science Funds of China [21005061]
  2. Specialised Research Fund for the Doctoral Programme of Higher Education of China [20096101120011]
  3. Natural Science Basic Research Plan in Shaanxi Province of China [2010JQ2013, 2012KJXX-25]
  4. Natural Science Foundation of Northwest University
  5. NWU Graduate Cross-Discipline Funds [10YJC19]

向作者/读者索取更多资源

A method based on electrogenerated chemiluminescence (ECL) for detection of DNA methylation and assay of the methyltransferase activity is developed, and it is demonstrated that the label-free ECL method is capable of detecting methyltransferase with a detection limit of 3 x 10(-6) U mL(-1), using a supersandwich amplification technique.

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