A dual-emission and large Stokes shift fluorescence probe for real-time discrimination of ROS/RNS and imaging in live cells

A novel dual-emission fluorescence probe has been developed for specific and sensitive detection of hypochlorite (ClO-). Upon addition of ClO-, significant changes in fluorescence emission intensity at two discrete wavelengths were observed. Meanwhile OONO- led to only a single-channel fluorescence enhancement. This feature makes it a clear advantage in distinguishing ClO-, RNS from other ROS.