期刊
CHEMICAL COMMUNICATIONS
卷 49, 期 18, 页码 1811-1813出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc38594a
关键词
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资金
- Ministry of Education, Culture, Sports, Science and Technology of Japan [20390032, 21310138]
- Grants-in-Aid for Scientific Research [21310138, 23390024, 20390032, 24658286] Funding Source: KAKEN
A photo-switchable fluorescent flagging approach has been developed to identify photoaffinity-labeled peptides in target protein. Upon photochemical release of the ligand, the protein was newly modified with a coumarin in place of the previously attached biotin. It allowed us to simplify complex identification processes for labeled sites.
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