期刊
CHEMICAL COMMUNICATIONS
卷 49, 期 26, 页码 2685-2687出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c3cc00077j
关键词
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资金
- National Institute of Standards and Technology-American Recovery and Reinvestment Act (NIST-ARRA)
- Intramural Research Program of the National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health
We have developed a method based on self-assembly of thiols on Au substrates to immobilize membrane proteins at interfaces. Using water soluble nitrilotriacetic acid (NTA)-terminated oligo(ethylene glycol) thiols, a histidine-tagged G protein-coupled membrane receptor (GPCR) was captured in a defined orientation with little nonspecific binding.
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