Technical note: Estimating aboveground plant biomass using a photographic technique

We present a non-destructive, photographic method to estimate biomass in semiarid grasslands. Though the method needs to be calibrated, it allows for a dramatic increase in the number of samples compared with the clipping method. The method is based on a relationship between the percentage of green pixels in a digital image and green biomass, We identified green pixels as those satisfying the following condition: G/B > 1 and G/R > 1, where G, B and R are the intensities of a particular pixel in the green, blue, and red bands respectively. The percentage of green pixels of the image and green grass biomass showed a correlation of 0.87 (n = 36, p < 0.001) when data were pooled from 3 sample dates, The relationship was slightly curvilinear and a log transformation of green biomass yielded a better correlation (r = 0.91, n = 36, p < 0.001), The percentage of green pixels showed a lower correlation with total green biomass than with grass biomass (r = 0.59 for the linear model and 0.73 for the log transformed model). The relationship between the percentage of green pixels and either green grass or total green biomass changed during the growing season. Both the slope and the Y-intercept of the model differed significantly among dates. Correlation coefficients for different dates ranged between 0.76 and 0.95.