Reaction-based two-photon probes for in vitro analysis and cellular imaging of monoamine oxidase activity

Reaction-based fluorescent probes for monoamine oxidases A and B are developed based on a new two-photon absorbing compound and its precursor. The probes show turn-on fluorescence response to the enzymes owing to the two-photon absorbing compound produced by the enzymatic activity, as monitored by one-as well as two-photon microscopy for the first time.