Supramolecular metal displacement allows on-fluorescence analysis of manganese(II) in living cells

Due to the importance of Mn2+ ions in biological processes, it is of growing interest to develop protocols for analysis of Mn2+ uptake and distribution in cells. A supramolecular metal displacement assay can provide ratiometric fluorescence detection of Mn2+, allowing for quantitative and longitudinal analysis of Mn2+ uptake in living cells.