期刊
CHEMICAL COMMUNICATIONS
卷 48, 期 79, 页码 9870-9872出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c2cc35028a
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资金
- DFG [MA 2288/12]
- FWF [I317]
- Austrian Science Fund (FWF) [I 317] Funding Source: researchfish
We present a screening assay based on fluorescence readout for the directed evolution of T7 RNA polymerase variants with acceptance of 2'-modified nucleotides. By using this screening we were able to identify a T7 RNA polymerase mutant with increased acceptance of 2'-methylseleno-2'-deoxyuridine 5'-triphosphate.
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