期刊
CELL CALCIUM
卷 27, 期 3, 页码 153-162出版社
CHURCHILL LIVINGSTONE
DOI: 10.1054/ceca.1999.0106
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- NHLBI NIH HHS [HL 057498] Funding Source: Medline
- NIGMS NIH HHS [GM 56686] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL057498] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM056686] Funding Source: NIH RePORTER
This study evaluated the relationship between regional elevation in intracellular calcium concentration ([Ca2+](i)) induced by acetylcholine (ACh) and the global cellular responses in porcine tracheal smooth muscle (TSM) cells. Regional (similar to 1.5 mu m(3)) and global (whole cell) changes in [Ca2+](i) were measured in fluo-3 loaded TSM cells using real-time confocal microscopy. Regional responses appeared as propagating [Ca2+](i) oscillations whereas global responses reflected the spatiotemporal integration of these regional responses. Within a region, [Ca2+](i) oscillations were 'biphasic' with initial higher frequencies, followed by slower steady-state oscillations. With increasing ACh concentration, the peak (maximum value relative to 0 nM) of regional [Ca2+](i) oscillations remained relatively constant, whereas both frequency and propagation velocity increased. In contrast, the global spatiotemporal integration of the regional oscillatory responses appeared as a concentration-dependent increase in peak as well as mean cellular [Ca2+](i). We conclude that the significance of ACh-induced [Ca2+](i) oscillations lies in the establishment of mean [Ca2+](i) level for slower Ca2+-dependent physiological processes via modulation of oscillation frequency and propagation velocity. (C) 2000 Harcourt Publishers Ltd.
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