期刊
CHEMICAL COMMUNICATIONS
卷 47, 期 40, 页码 11231-11233出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc14782j
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Tungsten oxidation worked as a simple chemical reaction for the effective detection of 5-hydroxymethylcytosine in DNA, distinguishing it from its epigenetic precursors, 5-methylcytosine and unmethylated cytosine. The tungsten-oxidation product obtained from 5-hydroxymethylcytosine was trihydroxylated thymine and was detected as a cleavage band in gel electrophoresis after treatment with hot piperidine.
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