期刊
BIOFOULING
卷 15, 期 1-3, 页码 13-23出版社
HARWOOD ACAD PUBL GMBH
DOI: 10.1080/08927010009386294
关键词
microbiologically influenced corrosion; Pseudomonas sp.; pit propagation
Pit propagation in carbon steel exposed to a phosphate-containing electrolyte required either stagnant conditions or microbial colonization of anodic regions. A scanning vibrating electrode (!;VE) was used to resolve formation and inactivation of anodic and cathodic sites on carbon steel. In sterile, continuously aerated medium, pits initiated and repassivated, while in the absence of aeration, Fits initiated and propagated. Pit propagation was also observed in continuously aerated medium inoculated with a heterotrophic bacterium, originally isolated from a corrosion tubercle formed on a steel pipe in a fresh water environment. Autoradiography of bacteria following uptake of C-14-acetate into cellular material in combination with SVE analysis demonstrated that sites of anodic activity coincided with sites of bacterial activity. Prelabeled bacteria also preferentially attached to corrosion products over the anodic sites. Confocal laser scanning microscopy demonstrated that attraction to anodic sites did not depend on bacterial viability and was not specific for iron as a substratum. The results suggest that bacteria may preferentially attach to the corrosion products formed over corrosion pits. The biofilms over these anodic sites may create stagnant conditions within corrosion pits that result in Fit propagation.
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