期刊
DEVELOPMENT GENES AND EVOLUTION
卷 210, 期 1, 页码 28-33出版社
SPRINGER VERLAG
DOI: 10.1007/PL00008185
关键词
random picking; in situ hybridization; medaka fish; development; ATPase; malate dehydrogenase
To rapidly isolate genes specifically expressed during medaka development we generated a cDNA library enriched for genes expressed in the head region of the developing embryo. Clones were spotted on filters automatically and preselected for abundantly expressed genes by hybridizing them with a probe derived from RNA of undifferentiated totipotent cells. Of the nonhybridizing clones 153 were chosen randomly and further analyzed by whole-mount in situ hybridization. There were 67 selected clones differentially expressed in the developing embryos, and 48 of these were expressed in the developing head, Differentially expressed genes were either of novel type or showed homology to known genes containing DNA binding motifs or to putative housekeeping genes.
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