期刊
CHEMICAL COMMUNICATIONS
卷 47, 期 15, 页码 4502-4504出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc00024a
关键词
-
资金
- National Key Basic Research Foundation of China [2010CB912300]
- National Natural Science Foundation of China [91013005]
A concise route was developed for the facile synthesis of a cyclic pyrrolysine analogue bearing an azide handle. Directed evolution enabled the encoding of this non-natural amino acid in both prokaryotic and eukaryotic cells, which offers a highly efficient approach for the site-specific protein labeling using click chemistry.
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