Analyte-induced formation of partial duplexes for the preparation of a label-free electrochemiluminescent aptasensor

Analyte-induced formation of partial duplexes was used for biosensor development with cocaine as a model. The cocaineaptamer interaction resulted in formation of a partial double strand section in the aptamer, where Ru(phen)(3)(2+) was intercalated for electrochemiluminescent analysis of cocaine.