A sensitive and selective resonance light scattering bioassay for homocysteine in biological fluids based on target-involved assembly of polyethyleneimine-capped Ag-nanoclusters

A specific resonance light scattering bioassay for homocysteine is developed on the basis of target-involved assembly of polyethyleneimine-capped Ag-nanoclusters. The bioassay permits discriminating homocysteine from cysteine, glutathione and other amino acids, and allows sensitive and selective detection of homocysteine with a detection limit of 42 nM.