9-Piperazine substituted perylene-3,4-dicarboximide as a fluorescent probe in ratiometric analysis

The communication describes the synthesis and studies of 9-piperazine substituted perylene-3,4-dicarboximide (1). In aqueous solution, the ratio of fluorescence emission intensities from the localized excited state (similar to 585 nm) to the charge transfer excited state (similar to 695 nm) increases in the pH range of 7.5-9. Furthermore, compound 1 may be used for ratiometric detection of double-stranded DNA.