期刊
CHEMICAL COMMUNICATIONS
卷 47, 期 19, 页码 5452-5454出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c1cc11114k
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资金
- RCUK
- EPSRC
- BBSRC
- Wellcome Trust
- UCLB
- Biotechnology and Biological Sciences Research Council [BB/H019332/1] Funding Source: researchfish
- Engineering and Physical Sciences Research Council [EP/E000754/1] Funding Source: researchfish
- BBSRC [BB/H019332/1] Funding Source: UKRI
- EPSRC [EP/E000754/1] Funding Source: UKRI
Controlling maleimide hydrolysis allows the modular construction of bromomaleimide-mediated bioconjugates which are either stable or cleavable in an aqueous, thiol-mediated reducing environment. The application of this methodology to reversible protein biotinylation, the irreversible labeling of peptide disulfide bonds and the assembly of stable, fluorescein-labelled glycoprotein mimics is described.
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