Facile synthesis of alumina hollow spheres for on-plate-selective enrichment of phosphopeptides

Alumina hollow spheres were synthesized, spotted and sintered on a stainless-steel plate. These spots were used to selectively capture phosphopeptides from peptide mixtures and the captured target peptides could be analyzed by MALDI-MS. This provides a fast and efficient on-plate selective enrichment method for phosphopeptides investigation.