期刊
LETTERS IN PEPTIDE SCIENCE
卷 7, 期 5, 页码 291-297出版社
SPRINGER
DOI: 10.1023/A:1011849912229
关键词
chemoselective ligation; Fmoc-methodology; iso-thiouronium salts; Kenner safety catch resin; native chemical ligation; peptide thioesters; peptide isomerisation; safety-catch linkers; solid-phase synthesis; thioester exchange
We describe, and compare, two methods which enable the assembly of proteins from peptide thioester fragments prepared by Fmoc chemistry mediated solid phase synthesis. The first, which utilizes iso-thiouronium salts, allows formation of thiophenyl esters directly from partially protected peptides, either in solution or on resin. The second uses `sulfonamide' based safety-catch resins. Data on yields, generality and potential for side-reactions are provided.
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