期刊
CLINICAL & EXPERIMENTAL METASTASIS
卷 18, 期 3, 页码 213-218出版社
KLUWER ACADEMIC PUBL
DOI: 10.1023/A:1006767405609
关键词
pancreatic cancer; metastasis; chronology; reporter gene; green fluorescence protein; fluorescence imaging; nude mice
类别
资金
- NCI NIH HHS [R44 CA 53963] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [R44CA053963] Funding Source: NIH RePORTER
Pancreatic cancer is a highly metastatic disease that responds poorly to currently-available treatment. In order to better visualize and understand the chronology and specificity of metastatic targeting of pancreatic cancer, two human pancreatic cancer cell lines, expressing green fluorescent protein (GFP), were studied in orthotopic models. MIA-PaCa2-GFP and BxPC-3-GFP tumor fragments were transplanted by surgical orthotopic implantation (SOI) to the nude mouse pancreas for fluorescence visualization of the chronology of pancreatic tumor growth and metastatic targeting. BxPC-3-GFP tumors developed rapidly in the pancreas and spread regionally to the spleen and retroperitoneum as early as six weeks. Distant metastases in BxPC-3-GFP were rare. In contrast, MIA-PaCa-2-GFP grew more slowly in the pancreas but rapidly metastasized to distant sites including liver and portal lymph nodes. Regional metastases in MIA-PaCa-2-GFP were rare. These studies demonstrate that pancreatic cancers have highly specific and individual 'seed-soil' interactions governing the chronology and sites of metastatic targeting.
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