期刊
CHEMICAL COMMUNICATIONS
卷 46, 期 3, 页码 469-471出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/b915843j
关键词
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We report a stepwise FLAG-tagging strategy for the purification of target proteins for bioactive metabolites. This method realizes the microscale purification and identification of target protein from as few as 1 x 10(5) differentiated cells. Using this method, we isolated and identified MetE as a cytosolic target protein of potassium isolespedezate, a metabolite controlling plant nyctinasty.
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