期刊
CHEMICAL COMMUNICATIONS
卷 46, 期 30, 页码 5461-5463出版社
ROYAL SOC CHEMISTRY
DOI: 10.1039/c0cc00735h
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资金
- Science and Engineering Research Council of A*STAR Singapore [0621010024]
We report the first example of directed evolution of a P450 monooxygenase with inverted enantioselectivity for asymmetric biohydroxylation. The biohydroxylation product of the best mutant 1AF4A has an ee of 83% (R) compared to the wild type's ee of 43% (S).
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