An ultrahighly sensitive and selective electrochemical DNA sensor via nicking endonuclease assisted current change amplification

Based on the nicking endonuclease assisted'' detection strategy, a novel electrochemical biosensor for the sequence-specific detection of DNA with ultrahigh sensitivity and selectivity is developed. By employing the above strategy, this DNA biosensor can detect as low as 0.068 fM target DNA and exhibits high discrimination ability even against a single-base mismatch.