期刊
BIOPHYSICAL JOURNAL
卷 79, 期 3, 页码 1655-1669出版社
BIOPHYSICAL SOCIETY
DOI: 10.1016/S0006-3495(00)76415-0
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- NINDS NIH HHS [1R01NS38129] Funding Source: Medline
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS038129] Funding Source: NIH RePORTER
Although reversible chemistry is crucial to dynamical processes in living cells, relatively little is known about relevant chemical kinetic rates in vivo. Total internal reflection/fluorescence recovery after photobleaching (TIR/FRAP), an established technique previously demonstrated to measure reversible biomolecular kinetic rates at surfaces in vitro, is extended here to measure reversible biomolecular kinetic rates of actin at the cytofacial (subplasma membrane) surface of living cells. For the first time, spatial imaging (with a charge-coupled device camera) is used in conjunction with TIR/FRAP. TIR/FRAP imaging produces both spatial maps of kinetic parameters (off-rates and mobile fractions) and estimates of kinetic correlation distances, cell-wide kinetic gradients, and dependences of kinetic parameters on initial fluorescence intensity. For microinjected rhodamine actin in living cultured smooth muscle (BC3H1) cells, the unbinding rate at or near the cytofacial surface of the plasma membrane (averaged over the entire cell) is measured at 0.032 +/- 0.007 s(-1). The corresponding rate for actin marked by microinjected rhodamine phalloidin is very similar, 0.033 +/- 0.013 s(-1). suggesting that TIR/FRAP is reporting the dynamics of entire filaments or protofilaments, For submembrane fluorescence-marked actin, the intensity, off-rate, and mobile fraction show a positive correlation over a characteristic distance of 1-3 mu m and a negative correlation over larger distances greater than similar to 7-14 mu m. Furthermore, the kinetic parameters display a statistically significant cell-wide gradient, with the cell having a fast and slow end with respect to actin kinetics.
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