期刊
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
卷 122, 期 8, 页码 1602-1608出版社
AMER CHEMICAL SOC
DOI: 10.1021/ja9926212
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We synthesized new type of diamide-CPI conjugate possessing a vinyl linker, 7. Sequence-selective alkylation of double-stranded DNA by 7 was investigated by high-resolution denaturing get electrophoresis using similar to 400 bp DNA fragments. Highly efficient alkylation predominantly occurs simultaneously at the purines of 5'-PyG(AIT)CPu-3' site on both strands at a nanomolar concentration of 7. These results suggest that the homodimer of conjugate 7 dialkylates both strands according to Dervan's pairing rule together with a new mode of recognition in which the Im-vinyl linker (L) pair targets G/C base pairs. In addition to the major dialkylation sites, a minor alkylation site was also observed at 5'-GT(PST)GC-3'. This alkylation can be explained by an analogous slipped homodimer recognition mode in which the L-L pair recognizes the A/T base pair. Efficient dialkylation by the homodimer of 7 was further confirmed using oligonucleotides (ODNs). HPLC analysis revealed that the conjugate 7 simultaneously alkylates GN3/AN3 of the target sequences on both strands of ODNs.
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