期刊
RADIOLOGY
卷 214, 期 3, 页码 795-800出版社
RADIOLOGICAL SOC NORTH AMER
DOI: 10.1148/radiology.214.3.r00mr34795
关键词
animals; annexin V; liver, radionuclide studies; liver, transplantation; mebrofenin; radionuclide imaging, experimental studies
资金
- NHLBI NIH HHS [HL61717, HL-47151] Funding Source: Medline
- NIDDK NIH HHS [DK47810] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL061717, P50HL047151] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF DIABETES AND DIGESTIVE AND KIDNEY DISEASES [R01DK047810, R29DK047810] Funding Source: NIH RePORTER
PURPOSE: To assess the value of imaging rejection-induced apoptosis with technetium 99m and annexin V, a human protein-based radiopharmaceutical used in the diagnosis of acute rejection of a liver transplant, in a well-characterized rodent model of orthotopic liver transplantation. MATERIALS AND METHODS: Tc-99m-radiolabeled annexin V was intravenously administered to six allografted (immunologically mismatched) and five isografted (immunologically matched) recipient rats on days 2, 4, and 7 after orthotopic liver transplantation. Animals were imaged 1 hour after injection of 0.2-2.0 mCi (8.0-74.0 MBq) of radiolabeled annexin V by use of clinical nuclear scintigraphic equipment. RESULTS: All animals in the allografted group demonstrated marked increases of 55% and 97% above the activity in the isografted group in hepatic uptake of annexin V on days 4 and 7, respectively. Severe acute rejection was histologically detected in all allografted livers on day 7. There was no histologic evidence of acute rejection in isografted animals. Dynamic hepatobiliary imaging with Tc-99m and mebrofenin, an iminodiacetic acid derivative, demonstrated no correlation with the presence or absence of acute rejection or with annexin V uptake. CONCLUSION: Noninvasive imaging with radiolabeled annexin V is more sensitive and specific than imaging with Tc-99m-mebrofenin in the diagnosis of acute rejection of a liver transplant.
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