4.7 Article

Specific N-terminal protein labelling: use of FMDV 3C(pro) protease and native chemical ligation

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CHEMICAL COMMUNICATIONS
卷 -, 期 29, 页码 3369-3371

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ROYAL SOC CHEMISTRY
DOI: 10.1039/b806727a

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  1. BBSRC [BB/D02014X/1] Funding Source: UKRI
  2. MRC [G0000138] Funding Source: UKRI
  3. Biotechnology and Biological Sciences Research Council [BB/D02014X/1] Funding Source: Medline
  4. Medical Research Council [G0000138] Funding Source: Medline

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We report an effective strategy for generating N-terminal cysteinyl proteins by proteolytic cleavage using the enzyme 3C(pro), suitable for a wide range of applications via native chemical ligation.

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