期刊
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
卷 48, 期 1, 页码 65-69出版社
AMER CHEMICAL SOC
DOI: 10.1021/jf990696j
关键词
fumonisins; ELISA; immunoassay; HPLC; extraction buffer comparison; corn
The Associatian of Official Analytical Chemists approved method for quantification of fumonisin B-1 (FB1) in corn meal or corn-based food products includes extraction into methanol (MeOH)/water (3:1, v/v). Disposal of the extraction medium can pose safety and environmental problems. To secure a rapid and inexpensive screen for FB1 contamination, a sensitive competitive ELISA using a rabbit polyclonal antibody was developed. This assay was used in a comparative study measuring the extraction efficiency of FB1 in aqueous or organic solvent buffers using 16 field corn samples. An aqueous phosphate buffer was found to be suitable for extracting FB1, thus eliminating the need for organic solvents. HPLC and ELISA determinations compared well in fortified samples at known concentrations between 1 and 50 mu g/mL of extract. Overestimation at levels >50 mu g/mL were common. The characteristics and application of the ELISA for screening purposes are discussed.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据