期刊
ANALYTICAL CHEMISTRY
卷 72, 期 1, 页码 52-60出版社
AMER CHEMICAL SOC
DOI: 10.1021/ac990878c
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资金
- NHGRI NIH HHS [R01HG00321, R01HG001808] Funding Source: Medline
- NIGMS NIH HHS [5T32GMO8349] Funding Source: Medline
- NATIONAL HUMAN GENOME RESEARCH INSTITUTE [R01HG000321, R01HG001808] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM008349] Funding Source: NIH RePORTER
A new mass spectrometric technique, charge reduction electrospray mass spectrometry (CREMS), allowing the analysis of complex mixtures of biological molecules is described. The charge state of ions produced by electrospray ionization may be reduced in a controlled manner to yield predominantly singly charged ions through reactions with bipolar (i.e., both positively and negatively charged) ions generated using a Po-210 ex particle source. The electrospray-generated multiply charged ions undergo charge reduction in a neutralization chamber positioned before the entrance nozzle to the mass spectrometer. The ions are detected using a commercial orthogonal electrospray time-of-flight mass spectrometer, although the neutralization chamber can be adapted to virtually any mass analyzer. The CREMS results obtained exhibit a signal intensity drop-off with Increasing oligonucleotide size similar to that observed with matrix-assisted laser desorption/ionization mass spectrometry, Proton-transfer reactions were found to be responsible for reducing charge on proteins and oligonucleotides in both positive and negative ion mode.
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