期刊
JOURNAL OF COLLOID AND INTERFACE SCIENCE
卷 229, 期 1, 页码 21-28出版社
ACADEMIC PRESS INC
DOI: 10.1006/jcis.2000.6992
关键词
thermodynamic incompatibility; air-water interface; competitive adsorption; phase separation; beta-casein; bovine serum albumin; binary protein films
Thermodynamic incompatibility and two-dimensional phase separation in a bovine serum albumin (BSA)/beta-casein/water ternary film at the air-water interface has been studied using an epifluorescence microscopy technique. The incompatibility between BSA and beta-casein at the air-water interface was deduced from deviation of the experimental equilibrium composition of the proteins in the mixed saturated monolayer film from that predicted by the Langmuir-type competitive adsorption model at various bulk concentration ratios. Fluorescence microscopy of the mixed monolayer film showed distinct phase-separated BSA-rich and beta-casein-rich regions coexisting with inhomogeneous mixed regions. BSA always tended to be the dispersed phase and beta-casein the continuous phase. It is suggested that because the free energy at the interfaces between the phase-separated regions is generally higher than at other regions of the film, they may act as zones of instability in protein-stabilized foams and possibly emulsions. (C) 2000 Academic Press.
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