期刊
AMERICAN JOURNAL OF PHYSIOLOGY-HEART AND CIRCULATORY PHYSIOLOGY
卷 279, 期 3, 页码 H901-H907出版社
AMER PHYSIOLOGICAL SOC
DOI: 10.1152/ajpheart.2000.279.3.H901
关键词
stress-activated protein kinases; curcumin; c-Jun amino-terminal kinase-1; c-Jun; p38 mitogen-activated protein kinase; adaptation; preconditioning; heart; anisomycin
资金
- NHLBI NIH HHS [HL-33889, HL-34360, HL-22559] Funding Source: Medline
- NATIONAL HEART, LUNG, AND BLOOD INSTITUTE [R01HL033889, R01HL034360, R01HL022559] Funding Source: NIH RePORTER
The role of stress-activated protein kinases (SAPKs), c-Jun NH2-terminal kinase (JNK) and p38 mitogen-activated protein (MAP) kinase, in preconditioning (PC) was examined with the use of isolated rat hearts subjected to four cyclic episodes of 5-min ischemia and 10-min reperfusion followed by 30-min ischemia and 2-h reperfusion (I/R). A group of hearts was preperfused with 100 mu M curcumin, a c-Jun and JNK1 inhibitor, or 5 mu M SB 203580, a p38 MAP kinase inhibitor. Another group of hearts was preperfused with 20 mu M anisomycin, a stimulator for both JNK and p38 MAP kinases. I/R increased the protein levels of JNK1, c-Jun, and p38 MAP kinase. PC also enhanced the induction of these kinases, but subsequent I/R-mediated increase was blocked by PC. Curcumin blocked I/R- and PC-mediated increase in JNK1 and c-Jun protein levels, whereas it had no effects on p38 MAP kinase. SB 203580, on the other hand, was equally effective in reducing the p38 MAP kinase activation but exerted no effects on JNK1 and c-Jun induction. I/R-mediated increased myocardial infarction was reduced by any of the following compounds: anisomycin, curcumin, and SB 203580. The cardioprotective effects of PC were abolished by either curcumin or SB 203580. The results demonstrate that PC is mediated by a signal-transduction pathway involving both JNK1 and p38 MAP kinase. Activation of SAPKs, although transient, is obligatory for PC.
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