期刊
MOLECULAR AND CELLULAR BIOLOGY
卷 20, 期 17, 页码 6354-6363出版社
AMER SOC MICROBIOLOGY
DOI: 10.1128/MCB.20.17.6354-6363.2000
关键词
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The p21-activated kinase PAK is targeted to focal complexes (FCs) through interactions with the SH3 domains of the PAK-interacting exchange factor PIX and Nck PIX is a Rac GTP exchange factor that also binds the G-protein coupled receptor kinase-interacting protein known as GIT1, Overexpression of GIT1 in fibroblasts or epithelial cells causes a loss of paxillin from FCs and stimulates cell motility. This is due to the direct interaction of a C-terminal 125-residue domain of GIT1 with paxillin, under the regulation of PM. In its activated state, GIT1 can promote FC disassembly independent of actin-myosin contractile events. Additionally, GIT directly couples to a key component of FCs, focal adhesion kinase (FAK), via a conserved Spa2 homology domain. We propose that GIT1 and FAK cooperate to promote motility both by directly regulating focal complex dynamics and by the activation of Rac.
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