期刊
EMBO JOURNAL
卷 19, 期 5, 页码 1098-1107出版社
WILEY
DOI: 10.1093/emboj/19.5.1098
关键词
Escherichia coli; 10Sa RNA; SsrA RNA; trans-translation
资金
- NATIONAL CENTER FOR RESEARCH RESOURCES [M01RR000042] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF ALLERGY AND INFECTIOUS DISEASES [R01AI011459, R01AI027837, R29AI027837] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [T32GM007315] Funding Source: NIH RePORTER
- NCRR NIH HHS [MO1-RR0042, M01 RR000042] Funding Source: Medline
- NIAID NIH HHS [AI11459-10, AI27837] Funding Source: Medline
- NIGMS NIH HHS [T32 GM007315] Funding Source: Medline
tmRNA, through its tRNA and mRNA properties, adds short peptide tags to abnormal proteins, targeting these proteins for proteolytic degradation. Although the conservation of tmRNA throughout the bacterial kingdom suggests that it must provide a strong selective advantage, if has not been shown to be essential for any bacterium. We report that tmRNA is essential in Neisseria gonorrhoeae. Although tagging per se appears to be required for gonococcal viability, tagging for proteolysis does not. This suggests that the essential roles of tmRNA in N. gonorrhoeae may include resolving stalled translation complexes and/or preventing depletion of free ribosomes. Although derivatives of N. gonorrhoeae expressing Escherichia coli tmRNA as their sole tmRNA were isolated, they appear to form colonies only after acquiring an extragenic suppressor(s).
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