Altered diffusion and perfusion in hydrocephalic rat brain: a magnetic resonance imaging analysis

Object. It can be inferred from data published in the literature that brain compression occurs in the early stages of acute hydrocephalus and that drainage of extracellular waste products is impaired. The authors hypothesized that compression of the cortex would alter water distribution and retard the diffusion of fluid in the hydrocephalic brain. Methods. Proton diffusion, blood perfusion, and T-1 and T-2 relaxation times were determined in adult rat brain by using magnetic resonance imaging prior to, and 1 and 8 days after induction of hydrocephalus by kaolin injection. Five anatomical regions of interest were studied. The striatum, dorsal cortex, and lateral cortex exhibited decreased T-2 and apparent diffusion coefficient (ADC) values bur no change in perfusion. Examination of white matter revealed an initial decrease in ADC followed by a significant increase. The T-2 relaxation times increased and perfusion decreased progressively between 1 and 8 days after induction of hydrocephalus. Conclusions. Acute experimental hydrocephalus causes compression of gray matter, perhaps associated with reduction in total water, which impairs diffusion of water in the tissue. White matter compression and hypoperfusion precede the development of edema. These findings have importance for understanding the neurochemical changes that occur in hydrocephalic brains.