期刊
FERTILITY AND STERILITY
卷 73, 期 3, 页码 479-486出版社
ELSEVIER SCIENCE INC
DOI: 10.1016/S0015-0282(99)00563-4
关键词
spermatozoa; motility; oviductal cells
Objective: To characterize in part the factor(s) in conditioned medium (CM) that maintains sperm motility after human oviductal cell culture. Design: Controlled, experimental, laboratory study. Setting: University-based gynecology unit. Patient(s): Fallopian tubes were obtained from patients who underwent tubal ligation or hysterectomy. Semen with normal sperm parameters was obtained from men who visited subfertility clinics. Intervention(s): Spermatozoa were incubated with CM and their motility was evaluated by a computer-aided sperm analysis system. Main Outcome Measure(s): Curvilinear velocity, straight-line velocity, average path velocity, linearity, amplitude of lateral head displacement, beat cross-frequency, and percentage of spermatozoa that exhibited hyperactivation. Result(s): Compared with their baseline motility (0 hour), spermatozoa incubated with CM maintained various motility parameters for a longer period than did control spermatozoa. All the motility parameters of the CM-treated spermatozoa were higher than those of the control spermatozoa at the same time point. This effect of CM was dose-dependent and increased with the duration of incubation. The effect was stable at 56 degrees C but was not observed after 100 degrees C heat treatment. Trypsin, but not proteinase K, abolished the effect. A fraction with a molecular weight of <3 kd in the CM was responsible for the observed effect. Conclusion(s): Human oviductal cells produce a peptide(s) that maintains sperm motility. (C) 2000 by American Society for Reproductive Medicine.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据