期刊
PHYTOCHEMISTRY
卷 55, 期 1, 页码 11-18出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0031-9422(00)00195-3
关键词
Pisum sativum; leguminosae; root meristems; histone deacetylase inhibitors; PAGE; protein profiles; Northern blots; Western blots
The histone deacetylase (HDA) inhibitors, trichostatin A (TSA) and HC toxin hall mitosis in cultured root meristems of Pisum sativum, while the anti-protozoan HDA inhibitor apicidin is ineffective. Two-dimensional PAGE of proteins from root meristems exposed to TSA and HC toxin did not show significant differences compared to controls, although a previously tested HDA inhibitor, butyrate, exhibited dramatic variations in its protein profile. Northern analysis of butyrate- and TSA-treated root meristems indicated that non-proliferating cells are expressing significant amounts of transcripts of the known cell proliferation associated genes: histone H2A, MAP kinase, cycA2:1 and cdc2. Western analysis reveals the presence of hyperacetylated nuclear proteins in HDA-inhibitor treated cells. These results suggest that the HDA inhibitors, butyrate and TSA, halt mitosis without down-regulating genes that typically have low or nonexistent expression levels in non-dividing cells. (C) 2000 Elsevier Science Ltd. All rights reserved.
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