期刊
CHINESE SCIENCE BULLETIN
卷 45, 期 17, 页码 1583-1586出版社
SCIENCE PRESS
DOI: 10.1007/BF02886217
关键词
fluorescent actin analogue; actin filaments; microinjection
Maize pollen actin has been labeled with Oregon Green 488 iodoacetamide. A yield of 3 mg fluorescent actin analogue has been obtained from 10 mg of maize pollen actin, which is 99% in purity and the dye/protein ratio is 72%. in the presence of Mg2+ and K+, the fluorescent actin analogue polymerized into filaments in vitro. Green fluorescent filaments were observed when the fluorescent actin was introduced into living plant cells by microinjection, indicating that the fluorescent actin analogue functions similarly to the native actin.
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