4.3 Article

Thermodynamics of DNA condensation caused by Mn2+ binding

期刊

CHEMICAL & PHARMACEUTICAL BULLETIN
卷 56, 期 3, 页码 247-249

出版社

PHARMACEUTICAL SOC JAPAN
DOI: 10.1248/cpb.56.247

关键词

thermodynamics; DNA condensation; isothermal titration calorimetry; supercoiled DNA; Mn2+ binding

向作者/读者索取更多资源

Interaction between Mn2+ ion and the two forms of DNA duplex (supercoiled and linearized pUC119 DNA) in solution has been examined by isothermal titration calorimetry. Although DNA condensation reaction heat was observed at 323 K, this was not the case at 298 K. DNA condensation was entropically driven and supercoiled DNA was found to be more susceptive. The enthalpy of DNA condensation is estimated 0.42 kJ/mol for both DNA forms. Conversely, the entropy of DNA condensation was 0.13 kJ/mol K for supercoiled DNA, and 0.12 kJ/mol K for linearized DNA. The difference of entropy is attributable to their DNA conformation.

作者

我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。

评论

主要评分

4.3
评分不足

次要评分

新颖性
-
重要性
-
科学严谨性
-
评价这篇论文

推荐

暂无数据
暂无数据