期刊
ELECTROCHIMICA ACTA
卷 45, 期 25-26, 页码 4373-4379出版社
PERGAMON-ELSEVIER SCIENCE LTD
DOI: 10.1016/S0013-4686(00)00544-2
关键词
biosensor; tyrosinase; phenols; carbon paste; electrode; electrocatalysis
Tyrosinase-based carbon paste electrodes are evaluated with respect to the viscosity and polarity of the binder liquids. The electrodes constructed using a lower viscosity mineral oil yielded a greater response to phenol and catechol than those using a higher viscosity oil of similar chemical composition. Despite its relatively high viscosity, the use of silicon oil Si PS 086 as carbon paste binder resulted in a 2-fold increase over Si AR 200 and a 4-6 fold increase (over mineral oils) in responses to phenol, p-chlorophenol, and p-cresol. Also reported is the enhanced enzyme electrode response to phenol and catechol resulting from pre-oxidation of these substrates using a plain carbon paste electrode in a series (in-line) dual electrode configuration. (C) 2000 Elsevier Science Ltd. All rights reserved.
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