期刊
NEURON
卷 27, 期 3, 页码 447-459出版社
CELL PRESS
DOI: 10.1016/S0896-6273(00)00056-8
关键词
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资金
- NINDS NIH HHS [NS-21624, NS-34045] Funding Source: Medline
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS034045, R01NS021624] Funding Source: NIH RePORTER
We constructed a novel optical indicator for chloride ions by fusing the chloride-sensitive yellow fluorescent protein with the chloride-insensitive cyan fluorescent protein. The ratio of FRET-dependent emission of these fluorophores varied in proportion to the concentration of CI- and was used to measure intracellular chloride concentration ([CI-](i)) in cultured hippocampal neurons. [CI-](i) decreased during neuronal development, consistent with the shift from excitation to inhibition during maturation of GABAergic synapses. Focal activation of GABA(A) receptors caused large changes in [CI-](i) that could underlie use-dependent depression of GABA-dependent synaptic transmission. GABA-induced changes in somatic [CI-](i) spread into dendrites, suggesting that [CI-](i) can signal the location of synaptic activity. This genetically encoded indicator will permit new approaches ranging from high-throughput drug screening to direct recordings of synaptic CI- signals in vivo.
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