期刊
AMERICAN JOURNAL OF CLINICAL PATHOLOGY
卷 113, 期 3, 页码 391-398出版社
AMER SOC CLIN PATHOLOGISTS
DOI: 10.1309/REV9-FDTM-5NGC-HBWY
关键词
hepatitis C; non-Hodgkin lymphoma; paraffin section; reverse-transcription double polymerase chain reaction; RT-PCR
类别
资金
- NATIONAL CANCER INSTITUTE [R01CA054576] Funding Source: NIH RePORTER
- NCI NIH HHS [CA 54576] Funding Source: Medline
Serologic resting shows that hepatitis C virus (HCV) may have a role in the pathogenesis of B-cell non-Hodgkin lymphomas (B-cell NHLs). We tried to demonstrate HCV RNA sequences in paraffin-embedded tissue from B-cell NHLs by reverse-transcription double polymerase chain reaction (RT-PCR) and Southern blotting. We studied 31 consecutive cases of B-cell NHLs; lymph nodes from 32 patients with diseases other than B-cell NHL were negative controls. Positive-strand HCV RNA was tested with primers for the 5' untranslated region. Replicative negative strand HCV RNA was tested with strand-specific RT-PCR for the 5' untranslated region. Immunohistochemical staining for HCV was done using an antibody to HCV core protein. Positive-strand HCV RNA was detected in 8 patients with B-cell NHL; negative-strand HCV RNA was detected in 6 of these cases, indicating viral replication. AII control cases were negative for HCV RNA. Immunohistochemistry, showed no staining of lymphoma cells for HCV core proteins in any case. HCV and B-cell NHLs may be associated. RT-PCR on paraffin-embedded lymphoma tissue is art alternative method of testing for HCV. The value of immunohistochemistry could nor be ascertained. The exact role of HCV in the pathogenesis of B-cell NHL needs to be studied further.
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