期刊
NEURON
卷 25, 期 3, 页码 611-623出版社
CELL PRESS
DOI: 10.1016/S0896-6273(00)81064-8
关键词
-
资金
- NATIONAL INSTITUTE ON AGING [R01AG013729, R01AG013730] Funding Source: NIH RePORTER
- NIA NIH HHS [AG-13730, AG-13729] Funding Source: Medline
The GDNF family ligands (GFLs: GDNF, neurturin, persephin, and artemin) signal through RET and a glycosyl-phosphatidylinositol (GPI)-anchored coreceptor (GFR alpha 1-alpha 4) that binds ligand with high affinity and provides specificity. The importance of the GPI anchor is not fully understood; however, GPI-linked proteins cluster into lipid rafts, structures that may represent highly specialized signaling organelles. Here, we report that GPI-anchored GFR alpha 1 recruits RET to lipid rafts after GDNF stimulation and results in RET/Src association. Disruption of RET localization using either transmembrane-anchored or soluble GFR alpha 1 results in RET phosphorylation, but GDNF-induced intracellular signaling events are markedly attenuated as are neuronal differentiation and survival responses. Therefore, proper membrane localization of RET via interaction with a raft-localized, GPI-linked coreceptor is of fundamental importance in GFL signaling.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据