期刊
EPILEPSIA
卷 41, 期 -, 页码 S40-S44出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1111/j.1528-1157.2000.tb06048.x
关键词
topiramate; epilepsy; Na+ channels; seizures; hippocampal neuron
资金
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R01NS023350, P01NS025630] Funding Source: NIH RePORTER
- NINDS NIH HHS [R01 NS 23350, P01 NS25630] Funding Source: Medline
Purpose: In this study, we examined the effects of topiramate (TPM) on the electrophysiologic properties of cultured rat hippocampal pyramidal neurons. Methods: Whole-cell current-clamp recording techniques were used to determine the effects of TPM on sustained repetitive firing (SRF), spontaneous epileptiform-burst firing, and spontaneous recurrent seizures (SRS). Results: Topiramate at therapeutic concentrations (10-100 mu M) significantly decreased or abolished SRF in a dose-dependent and partially reversible manner. When transiently exposed to a medium in which Mg2+ is omitted, hippocampal neurons in culture develop SRS (epilepsy) and epileptiform discharges. Application of TPM at concentrations ranging from 10 to 100 mu M to cells displaying seizure activity caused a concentration-dependent decrease in the number of action potentials within a burst and in the average duration of epileptiform activity. Both effects were partially reversed during a 5- to 30-min drug washout period. Conclusions: These effects on the electrophysiologic properties of cultured neurons are consistent with the concept that TPM exerts modulatory effects on voltage-dependent Na+ and/or Ca2+ conductances responsible for the generation and propagation of action potentials. Topiramate also may inhibit synaptic conductances responsible for transmission of epileptiform discharges.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据