期刊
CHROMATOGRAPHIA
卷 51, 期 -, 页码 S139-S142出版社
SPRINGER HEIDELBERG
DOI: 10.1007/BF02492796
关键词
capillary electrochromatography; octadecyl silica; carbohydrate analysis; monosaccharides; derivatisation
A packing procedure was adopted for capillary electrochromatography (CEC) that produces capillary columns with high separation efficiencies. The columns were fully packed, 50 cm long, with UV detection being performed through the packed section 30 cm from the inlet end. The CEC experiments were run at ambient pressure, with no additional pressure applied to the ends of the column. The stationary phase (octadecyl silica (ODS), 5 mu m) promoted a high velocity electroosmotic flow (EOF), enabling rapid and efficient separation of a hydrocarbon test mixture. Some 1-phenyl-3-methyl-5-pyrazolone (PMP) derivatives of mono- and disaccharides were baseline separated, using a 5 mM NaH2PO4 in 80% acetonitrile and 20% water (v/v) buffer solution. CEC shows promise for future applications in carbohydrate analysis.
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