期刊
EPILEPSIA
卷 41, 期 -, 页码 S14-S17出版社
LIPPINCOTT WILLIAMS & WILKINS
DOI: 10.1111/j.1528-1157.2000.tb01550.x
关键词
hippocampus; spine loss; spine regeneration; mossy fiber sprouting; spontaneous seizure
资金
- NINDS NIH HHS [NS02808, NS31180] Funding Source: Medline
- NATIONAL INSTITUTE OF NEUROLOGICAL DISORDERS AND STROKE [R29NS031180, P01NS002808] Funding Source: NIH RePORTER
Purpose: To study when dendritic alteration can occur in the epileptic hippocampus and how it is influenced by epileptic axonal reorganization. Methods: Human specimens and the rat pilocarpine model were used. Dentate granule cells (DGCs) were visualized by intracellular biocytin injection for spine count. Results: In the rat pilocarpine model, dendrites of DGCs revealed a generalized spine loss immediately after the acute status epilepticus induced by pilocarpine. However, this generalized damage was transient and was followed by recovery and plastic changes in spine shape and density, which occurred 15 to 35 days after the initial acute status, i.e., during the period of establishing a chronic phase of this model with the induction of spontaneous seizures. In human epileptic hippocampi, spine density was significantly higher when DGCs generated aber-rant mossy fiber collaterals. This was particularly so in the proximal dendrite of DGCs, where the aberrant collaterals were densely localized. These findings suggest that initial acute seizures do not cause permanent damage in dendrites and spines of DGCs and that dendritic spines of epileptic neurons can respond to changes in the local cellular environment, including newly formed afferents, in a plastic manner. Conclusion: Dendritic spines are dynamically maintained in chronic epilepsy during the course of establishment and maintenance of spontaneous seizures. Local dendritic spine alteration, detected later in the chronic phase of epilepsy, must have a separate cause from initial acute insults.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据