期刊
MOLECULAR CELL
卷 6, 期 3, 页码 573-582出版社
CELL PRESS
DOI: 10.1016/S1097-2765(00)00056-3
关键词
-
资金
- NCI NIH HHS [5T32CA09139] Funding Source: Medline
- NIGMS NIH HHS [GM53824] Funding Source: Medline
- NATIONAL CANCER INSTITUTE [T32CA009139] Funding Source: NIH RePORTER
- NATIONAL INSTITUTE OF GENERAL MEDICAL SCIENCES [R01GM053824] Funding Source: NIH RePORTER
We report that the bacterial transposon Tn7 can preferentially transpose into regions where chromosomal DNA replication terminates. DNA double-strand breaks are associated with the termination of chromosomal replication; therefore, we directly tested the effect of DNA breaks on Tn7 transposition. When DNA double-strand breaks are induced at specific sites in the chromosome, Tn7 transposition is stimulated and insertions are directed proximal to the induced break. The targeting preference for the terminus of replication and DNA double-strand breaks is dependent on the Tn7-encoded protein TnsE. The results presented in this study could also explain the previous observation that Tn7 is attracted to events associated with conjugal DNA replication during plasmid DNA transfer.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据