期刊
PLANT AND SOIL
卷 221, 期 1, 页码 33-38出版社
SPRINGER
DOI: 10.1023/A:1004739621352
关键词
ammonium; birch; gene expression; nia promoter; nitrate; nitrate reductase
A 1535 bp promoter of the nitrate reductase gene (nia) from birch (Betula pendula) and a series of 5' deletions were fused to the beta-glucuronidase (GUS) gene and introduced into Nicotiana plumbaginifolia. In transgenic plants the NR promoter sequences directed strong GUS expression in the root epidermal hair cells, and in phloem cells of leaf and stem vascular tissue. The NR promoter confers also a significant stimulation of the GUS gene expression by nitrate. These findings might indicate that nitrate flow is one of the signals involved into tissue and cell specific expression of the NR promoter GUS fusions.
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